载脂蛋白E基因启动子-307～-7区单核苷酸多态性与散发阿尔茨海默病的相关研究

A study on the relation between single nucleotide polymorphism of the apolipoprotein E promoter spanning-307 to-7 and sporadic Alzheimer's disease

目的探讨载脂蛋白E(ApoE)基因启动子-307～-7区单核苷酸多态性(SNP)与散发阿尔茨海默病(SAD)发病风险的关系。方法在36例SAD患者和36例正常人中,采用聚合酶链反应和变形梯度凝胶电泳检测再测序的方法,对ApoE基因启动子-307～-7区进行SNP的检测。结果ApoE基因启动子-307～-7区存在-219G/T多态,未发现新的SNP,SAD患者与正常对照组之间基因型频率分布无差异(P<0.05)。结论ApoE基因启动子-307～-7区未发现新的SNP,-219G/T多态与SAD无关。

Objective To explore the association between single nucleotide polymorphism of the apolipoprotein E （ApoE） promoter span- ning - 307 to - 7 and sporadic Alzheimer＇s disease （SAD）. Methods We detected single nucleotide polymorphism of the apolipoprotein E promoter spanning - 307 to - 7 in 36 SAD and 36 healthy subjects with polymerase chain reaction and denaturing gradient gel electrophoresis （DGGE） followed by sequencing. Results We found no distribution differences of the ApoE promoter -219 G/T polymorphism between SAD and controls （ P 〈 0.05）, and no new single nucleotide polymorphism in the ApoE promoter spanning - 307 to - 7. Conclusion The ApoE promoter -219 G/T polvmorphism is not correlated to the risk for SAD.