长链二羧酸在Bioflo-Ⅱ型发酵罐中产生的研究

Studies on the Production of Long-chain Dicarboxylic Acids in the Bioflo- Ⅱ Bioreactor

热带假丝酵母（Ｃａｎｄｉｄａｔｒｏｐｉｃａｌｉｓ）突变株ＳＤ１，ＧＵ１１，ＬＤ１２，ＹＵｍ和ＨＹＵｍ 分别被用来发酵不同链长的烷烃生产二羧酸。在 ６．６Ｌ Ｂｉｏｆｌｏ－Ⅱ发酵罐上对它们的产酸 条件进行了试验。采用流加烷烃等方法使产酸量大大提高。１，１２—十二碳二羧酸的产量 最高，质量浓度达 １４７ｇ／Ｌ。混合二羧酸也达到 １４０ ｇ／Ｌ。耐高温菌株 ＨＹＵｍ在 ３６℃下 生产１，１３—十三碳二羧酸，达８８．９ｇ／Ｌ。实验成功地获得了从癸二酸到１，１７—十七碳 二羧酸及混合二羧酸共９种二羧酸并研究了它们生产方法。

Candida tropicalis mutant SD1, GU11, LD12,YUm and HYUm were used to ferment alkanes of different chain length to produce dicarboxylic acids. The DC producing conditions were tested in a 6.6 L Bioflo- Ⅱ bioreactor. The DC yield was enhanced by method of alkane supplementation. The highest DC yield was that of dodecandioc acid, which run as high as 147 g/L.The yield of mixed dicarboxylic acids was 140 g/L. The thermo tolerant mutant HYUm produced tridecandioic acid at 36℃, its yield reached 88.9 g/L.Nine kinds of dicarboxylic acids from DC10 through DC17 and DCm were successfully produced.