丹参对实验糖尿病大鼠粘附分子和坐骨神经的影响

Influence of Salvia on sciatic nerve and the level of ICAM-1 in STZ-induced diabetic rats

目的：研究丹参对糖尿病大鼠体内两种粘附分子-细胞间粘附分子-1（ICAM-1）和P-选择素（P-selectin）表达的影响和对坐骨神经超微结构及传导速度的影响；方法：将SD大鼠随机分正常、糖尿病和丹参3组，测定各组血糖、糖化血红蛋白、单个核细胞表面ICAM-1和血小板表面P-selectin表达水平；观察坐骨神经超微结构及测定其传导速度。结果：丹参能够明显降低单个核细胞表面ICAM-1的表达（丹参=351．0％±5．16VSDM=65．66％±11．10，P〈0．05），但对血小板P．selectin的表达影响不明显。坐骨神经超微结构的病理变化明显改善，传导速度明显加快（丹参=39．50m／s±4．07VSDM=19．76m／s±3．21，P〈0．05）；而且ICAM-1表达的下降与坐骨神经传导速度及超微结构的病理变化明显改善存在显著的相关性。结论：丹参能降低糖尿病大鼠体内ICAM-1水平，并改善了糖尿病引起的坐骨神经病理和传导速度的功能变化。

Objectives： To study the level of ICAM-1 on the surface of mononuclear cell and P-selectin on the platelet in the STZ-induced Diabetic rats and the effect on the ultrastructure and conductive velocity of Sciatic nerve by salvia miltiorrhiza bge treatment. Methods The SD rats were randomly divided into three groups： normal control, diabetes control, salvia mlltiorrhiza bge. Sciatic nerve conductive velocity, Blood glucose, HbAlc, the level of ICAM-1 on the surface of mononuclear cell and P-selectin on the platelet were examined, ultrastructure of sciatic nerve were observed. Results Salvia miltiorrhiza beg increased sciatic nerve conductive velocity significantly （DM=19.76m/s± 3.21 vs Salvia miltiorrhiza beg=39.50m/s±4.07, P〈0.05）, improved ultrastructure of sciatic nerve pathological changes, decreased the level of ICAM-1 on the surface of mononuclear cells （Salvia miltiorrhiza beg=35.10%±5.16 vs DM=65.66%±11.10, P〈0.05 ） on Diabetic rats, but had no significant effect on P-selectin on the surface of platelets. Conclusion The study suggests that salvia miltiorrhiza bge can improve pathological changes of Sciatic nerve as well as decreasing expression of ICAM- 1 in DM-rats, and delay the development of diabetic neuropathy.