牙周炎大鼠血清和牙龈组织中白细胞介素-6含量比较分析

Comparative analysis of the levels of IL-6 in blood serum and gingival tissue of experimental periodontitis rats

目的通过检测牙周炎大鼠血清及牙龈组织中白细胞介素-6(IL-6)含量的变化,探讨IL-6在牙周炎病理进程中的作用。方法2005年5—10月选取纯种雌性SD大鼠72只,随机分为对照组和实验组,各组36只。对照组大鼠不作任何处理;实验组大鼠行单纯牙周结扎,建立牙周炎动物模型。术后第4、6、8周各组随机抽取11只SD大鼠,采集血液标本,处死后切取上颌骨及牙龈组织,制作切片进行组织学观察,并测定血清和牙龈组织中IL-6的含量。结果牙龈组织中的IL-6含量在第4、6、8周明显高于对照组(P<0.05)。血清中的IL-6的含量在第4、6周与对照组相比差异无统计学意义(P>0.05),第8周时明显高于对照组,且差异有统计学意义(P<0.05)。结论IL-6的含量与牙周组织破坏程度直接相关,在牙周炎治疗中及时控制牙龈组织中IL-6合成具有临床应用价值。

Objective Through observing the change of in terleukin-6（IL-6） in the blood serum and gingival tissue,study the possible role of IL-6 in the development of periodontitis.Methods Seventy-two SD rats were randomly divided into two groups of thirty-six（group N,group P）.The periodontitis model was made.At the 4th,6th,8th week after the animal models were established,the rats were killed respectively and the maxilla and the peripheral blood were collected.The sections were made for histological examination and IL-6 was determined in the serum and gingival tisues.Results IL-6 concentration in gingival tissues was significantly higher in periodontitis group at the 4th,6th and 8th week than that in the counterpart controls（P 0.05）.IL-6 concentration in blood serum had no significant change among these two groups（P0.05） at the 4th and 6th week,but at the 8th week,it was more significantly increased than that in the counterpart controls（P 0.05）.Conclusion IL-6 concentration is related to the damage of periodontal tissues,Reducing the IL-6 level in gingival tissue is helpful in the healing of periodontal tissues.