哮喘患者外周血淋巴细胞DNA的损伤及意义

Significance of DNA damage in peripheral blood lymphocytes of patients with asthma

目的探讨哮喘患者外周血淋巴细胞DNA的损伤及意义。方法选取32例哮喘患者和30例健康对照者,其中哮喘急性发作期15例,缓解期17例,采用胞质分裂阻断微核(CBMN)法观察外周血淋巴细胞的DNA损伤。结果哮喘患者组的微核率、核芽率、染色体桥率分别为(26.34±6.79)‰、(2.99±1.66)‰、(7.68±5.26)‰;健康对照组的微核率、核芽率、染色体桥率分别为(13.16±3.81)‰、(1.72±0.64)‰、(4.59±2.22)‰;微核率、核芽率、染色体桥率两组比较差异有统计学意义(P<0.01或<0.05)。哮喘急性发作期和缓解期患者微核率比较差异无统计学意义(P>0.05)。哮喘患者微核率与病程有相关性(rs=0.424,P<0.05)。结论哮喘患者外周血淋巴细胞存在DNA的损伤,可能与哮喘的气道慢性炎症及氧化应激相关。

Objective To explore the condition of DNA damage in the peripheral blood lymphocytes of patients with asthma. Methods Thirty two asthmatic patients（15 cases of asthmatic patients with acute exacerbation and 17 cases of asthmatic patients in remission stage） and 30 controls were enrolled. This study evaluated the condition of DNA damage of the peripheral blood lymphocytes using the cytokinesis-blocked micronucteus（CBMN）. Results The mean frequency of micronuelei in patients with asthma and the control group was （26.34 ± 6.79） ‰ and （ 13.16 ± 3.81 ）‰ respectively（ P 〈0.01）. The frequency of nuclear buds in patients with asthma was （2.99± 1.66）‰,and in control group,（1.72± 0.64）‰ （ P 〈0.01）. The frequency of nucleoplasmic bridges was （7.68 ± 5.26）‰ in patients with asthma and （4.59±2.22）‰ in the control group（ P 〈0.05）. The micronuclei rates in acute exacerbation group and asthma remission group did not differ significantly（ P 〉0.05）. The frequency of micronuclei in patients with asthma was correlated with disease progression （ rs= 0. 424, P 〈 0.05）. Conclusion DNA damage exists in the peripheral blood lymphocytes of asthma patients, and it may be associated with airway chronic inflammation and oxidative stress of asthmatic patients.