抗线虫基因番茄秸秆对土壤生物活性的影响

Effects of Tomato Straw with Nematode-resistance Gene on Soil Biological Activities

采用室内模拟培养的方法,研究了不同用量(2%,4%)转抗线虫基因番茄秸秆和普通番茄秸秆分解对土壤酶活性及微生物量碳的影响。结果表明:与普通番茄秸秆相比,添加低量(2%)秸秆,23d前,抗线虫处理土壤脲酶活性显著提高,9d时土壤碱性磷酸酶活性显著提高,23d时则显著降低,23d后,土壤微生物量碳含量显著提高。添加高量(4%)秸秆,4d时抗线虫处理土壤脲酶活性显著提高,91d时则显著降低,9d时,土壤碱性磷酸酶活性显著提高,23d,42d时则显著降低,9d,23d时,抗线虫处理土壤微生物量碳含量显著提高。抗线虫处理与普通处理间土壤蔗糖酶活性的差异在秸秆添加量为2%和4%时都不显著。

Simulated experiment was conducted to study effects of decomposition of tomato straw with nematode-resistance gene on soil enzyme activities and microbial biomass carbon in comparison with ordinary tomato straw in different application amount （2%,4%）.The results showed that：after adding low amount of straw （2%）,before day 23,soil Urease activity was significantly higher in nematode resistance treatments than in ordinary treatments.Soil alkaline phosphatase activity in nematode resistance treatments was significantly increased on day 9,but decreased on day 23.After incubation for 23 days,soil microbial biomass carbon was significantly higher in nematode resistance treatments than in ordinary treatments.After adding high amount of straw （4%）,soil Urease activity in nematode resistance treatments was significantly increased on day 4,but decreased on day 91.Soil alkaline phosphatase activity in nematode resistance treatments was significantly increased on day 9,but decreased on day 23 and day 42.On day 9 and day 23,soil microbial biomass carbon was significantly higher in nematode resistance treatments than in ordinary treatments.Whether applied low or high amount of straw,there was no significant difference in soil invertase activity between the two treatments.