益生菌对犬异位性皮炎免疫调节机制的研究

Immunoregulatory effect of probiotic on canine atopic dermatitis

本试验对使用益生菌治疗的21只异位性皮炎犬和9只健康犬进行调查,通过流式细胞术检测其外周血淋巴细胞FoxP3表达(即Treg的生成),ELISA试剂盒检测血清IL-10、TGF-β、IFN-γ和IgE浓度,并比较治疗前后患犬的瘙痒程度。结果表明,服用特殊益生菌使犬外周血淋巴细胞FoxP3表达升高,血清IL-10和IgE浓度分别升高和下降。且通过提高Treg细胞的数量控制异位性皮炎的发病。

The present study was designed to investigate the generation of regulatory T cells（Treg） and cytokines IL-10,TGF-β,IFN-γ and immunoglobulin E,in peripheral lymphocytes in 21 atopy canines treated with probiotics compared to 9 healthy control dogs.The results showed that the proportion of FoxP3-positive peripheral lymphocytes was significantly increased,and the concentrations of IL-10 and IgE in the serum of dogs treated with probiotics were increased and decreased as compared to controls respectively,suggesting a role of probiotics in the treatment of atopic dermatitis.