番木瓜中皂苷类成分的研究

Analysis of Saponins from Carica papaya Pulp

目的:研究番木瓜果肉的化学成分,寻找活性物质。方法:用85%乙醇提取,大孔吸附树脂、ODS、MCI柱层析进行分离,NMR波谱法鉴定化合物的结构。结果:从番木瓜果肉乙醇提取物中分离到3种化合物,分别为2β,3β-二羟基-乌苏酸(Ⅰ)、3-O-葡萄糖-甾苷(Ⅱ)、薯蓣皂苷元-3-O-β-D-吡喃葡萄糖基(1→3)-β-D-吡喃葡萄糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Ⅲ)。

Objectives：To investigate bioactive compounds from Carica papaya pulp.Methods：Chemical components in Carica papaya pulp was extracted using 85% ethanol and purified by marcoporous resin adsorption followed by ODS and MCI column chromatography.The structures of these compounds were elucidated through NMR spectroscopic analysis.Results：Three compounds were isolated from Carica papaya pulp and their chemical structures were identified as 2β,3β-dihydroxy-ursolic acid（Ⅰ）;3-O-glycoside-sterol（Ⅱ）;diosgenin-3-O-β-D-glucopyranosyl（1 → 3）-β-D-glucopyranosyl（1 → 4）-[α-Lrhamnopyranosyl（1→2）]-β-D-glucopyranoside（Ⅲ）,respectively.Conclusion：To the best of our knowledge,this paper is the first one that reports compounds I-Ⅲ obtained from Carica papaya pulp.