高压静电场对青霉菌的影响

Effect of High Voltage Electrostatic Field on Sporulation and Germination of Penicillium Spores

以苹果霉腐病中青霉菌为材料通过高压静电场(HVEF)进行不同时间的处理,比较处理的孢子菌悬液在不同培养温度下的萌发和产孢量,将处理后的孢子菌悬液接种于苹果,观察苹果的感病率及病斑直径。结果表明:同一温度下培养,高压静电场处理的与未处理菌悬液的孢子萌发率相差不大,但萌发时间滞后;产孢量与处理时间相关,处理30min后,在20℃培养7d产孢量要比处理15min和未处理的低。与对照相比,接种处理的孢子菌悬液的苹果感病率较低,病斑直径较小。

Penicillium as one of the microorganisms causing apple mouldy core and core rot was subjected to high voltage electrostatic field（HVEF） treatment for different time to compare sporulation and germination of treated Penicillium spores during suspension culture at different temperatures.Meanwhile,the treated Penicillium spore suspension was inoculated into wounds in apples and infection rate and lesion diameter were measure every day during storage at 25 ℃.Results showed that no obvious difference in germination rate was observed between Penicillium spores with and without HVEF treatment during suspension culture at the same temperatures.However,the germination time of Penicillium spores was delayed due to HVEF treatment.In addition,sporulation had an obvious relationship with the treatment time of HVEF.Compared with Penicillium spore suspension treated with HVEF for 15 min or without FVEF treatment,Penicillium spore suspension with 30 min of HVEF treatment exhibited a lower sporulation after 7 days of culture at 20 ℃.Therefore,lower infection rate and smaller lesion diameter in apple inoculated with Penicillium spore suspension treated with HVEF were observed.