新疆芳香和帕米尔新塔花精油组成及其抗氧化的研究(英文)

Essential Oil Compositions and Antioxidant Activities of Two Ziziphora Species in Xinjiang

优化水蒸气蒸馏法提取新疆芳香和帕米尔两种新塔花挥发油的最佳条件,利用气相色谱-质谱联用技术分析两种新塔花挥发油的化学成分和组成,研究两种新塔花挥发油的抗氧化能力。从芳香新塔花的地上部分提取的挥发油中分离鉴定出10种成分,占挥发油总量的99.7%以上。芳香新塔花的主要成分为长叶薄荷酮(80.7%)、薄荷酮(8.3%)、胡椒烯酮(4.9%)及异薄荷酮(2.6%)。从帕米尔新塔花的地上部分提取的挥发油中分离鉴定出29种成分,占挥发油总量的99.8%以上。帕米尔新塔花精油中主要含有长叶薄荷酮(45.9%)、异薄荷酮(24.1%)、(+)-新薄荷醇(10.1%)和百里香酚(9.4%)。通过与百里香酚,VC和BHT的对比,新塔花精油具有一定的抗氧化能力,能够有效清除DPPH·,超氧阴离子自由基和羟自由基;能够保护脂质体,抑制其过氧化;并且具有明显的清除亚硝酸盐的作用。结果表明,帕米尔新塔花精油的抗氧化效果优于芳香新塔花精油。

The chemical composition of essential oils isolated by hydrodistillation from the aerial parts of Ziziphora clinopodioides Lam.and Ziziphora pamiroalaica Juz.were analyzed by gas chromatography/mass spectrometry（GC-MS）.Ten constituents representing 99.7% of the Z.clinopodioides oils were identified,which contained（＋）-（R）-Pulegone（80.7%）,Menthone（8.3%）,Piperitenone（4.9%） and（＋）-Isomenthone（2.6%） as the major components;On the other hand,twenty-nine constituents representing 99.8% of the oils from Z.pamiroalaica.were identified,the major compounds consisted of（＋）-（R）Pulegone（45.9%）,（＋）-Isomenthone（24.1%）,（＋）-Neomenthol（10.1%）,Thymol（9.4%）.The antioxidant activity of the oils was assessed by measurement of the free radical scavenging（DPPH） activity,the reductive potential,Superoxide anion scavenging activity,NaNO2 scavenging ability and by the TBARS assay,compared with that of synthetic antioxidants：Thymol,Vitamin C（VC） and butylated hydroxytoluene（BHT）.Results showed that the oil from Z.pamiroalaica.was better than that from Z.clinopodioides in antioxidant abilities.