摘要
从不同品牌的10种酸乳中分离得到10株德氏乳杆菌(Lactobacillus delbrueckii),经生理生化实验及特异性PCR鉴定,L.d3、L.d4、L.d5、L.d6、L.d8、L.d10为德氏乳杆菌保加利亚亚种(Lactobacillus delbrue ckii subsp. bulgaricus),L.d1、L.d2、L.d7、L.d9为德氏乳杆菌乳酸亚种(Lactobacillus delbrueckii subsp. lactis)。利用24个随机引物对上述10株德氏乳杆菌进行随机扩增DNA多态性分析,并使用NTSYSpc-2.10e软件对扩增图谱数据进行处理,得出10个菌株之间的遗传相似系数矩阵及聚类分析图。结果表明,10个菌株相互间的遗传相似系数在0.4167～0.8833之间,不同菌株间存在一定的亲缘关系及遗传异质性。
Ten strains of Lactobacillus delbrueckii were isolated from ten commercial brands of yogurts.L.d 3,L.d 4,L.d 5,L.d 6,L.d 8 and L.d 10 were identified as Lactobacillus delbrueckii subsp.bulgaricus,and L.d 1,L.d 2,L.d 7 and L.d 9 were identified as Lactobacillus delbrueckii subsp.lactis according to physiological and biochemical tests and specific PCR identification.The genetic diversity of ten strains was evaluated through random amplified polymorphic DNA（RAPD） analysis using 24 random primers,and the similarity coefficient matrix and clustering analysis were achieved according to statistical analysis of fingerprinting data using NTSYSpc-2.10e software.Results indicated that genetic similarity coefficients of ten strains were ranged in 0.4167 and 0.8833.The genetic relationship and heterogeneity existed among different strains to a certain extent.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第7期177-181,共5页
Food Science
基金
"十一五"国家科技支撑计划项目(2006BAD04A14)
