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酒酒球菌抗酸相关基因RAPD标记的筛选 被引量：1

Screening of RAPD Markers Linked to Acid Resistance-related Gene in Oenococcus oeni
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摘要 对影响RAPD反应体系的主要因素进行优化,建立适合本实验室的RAPD反应体系。在此基础上以33株抗酸性酒酒球菌和9株酸敏性菌株为试材,采用BSA法和RAPD技术,通过对45个随机引物的筛选以及在单菌株中的筛选验证,最终获得与酒酒球菌抗酸性状相关的3个RAPD标记:S40-1400、S333-2500、S333-650。 Major factors affecting RAPD reaction system were optimized and an optimal RAPD reaction system was established for laboratory investigations.Subsequently,33 acid-resistant Oenococcus oeni strains and 9 acid-sensitive Oenococcus oeni strains were used as experimental materials for screening RAPD markers linked to acid resistance-related genes.Random amplified polymorphic DNA（RAPD） and bulked segregate analysis（BSA） techniques were employed in this study.Three RAPD markers S40-1400,S333-2500 and S333-650 linked to acid resistance in Oenococcus oeni were obtained through a selection from 45 random primers and confirmation in a single Oenococcus oeni strain.
作者 刘树文 李西柱 王坐京 周立国
机构地区 西北农林科技大学葡萄酒学院 陕西省葡萄与葡萄酒工程技术研究中心
出处 《食品科学》 EI CAS CSCD 北大核心 2010年第7期190-195,共6页 Food Science
基金 现代农业产业技术体系建设专项资金资助项目(nycytx-30-ch-03) 农业部"948"项目(2009-Z29)
关键词 酒酒球菌 抗酸相关基因 RAPD标记 Oenococcus oeni acid-resistant gene RAPD markers
分类号 Q93-3 [生物学—微生物学] Q78 [生物学—分子生物学]
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参考文献20

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二级参考文献55

共引文献371

同被引文献14

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食品科学
2010年 第7期

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