大球盖菇提取物对CCl_4所致急性肝损伤小鼠的抗氧化酶活性及同工酶的影响

Effect of Stropharia rugoso-annulata Extract(SRE) on Activities and Isozyme Profiles of Antioxidant Enzymes in Mice with Carbon Tetrachloride-induced Acute Liver Injury

目的:研究大球盖菇提取物(SRE)对小鼠肝和主要脏器的抗氧化酶活性及同工酶的影响。方法:小鼠连续30d给予低(100mg/(kgbw·d))、中(200mg/(kgbw·d))、高(400mg/(kgbw·d))剂量SRE后,灌胃给予CCl4(80mg/(kgbw·d))建立动物急性肝损伤模型,动物处死后取肝及主要脏器测定抗氧化酶活性,采用聚丙烯酰胺梯度凝胶电泳分析CAT和SOD同工酶。结果:与模型组比,高剂量SRE能显著提高肝总SOD、Mn-SOD、CuZn-SOD及CAT、GPx活性。主要脏器抗氧化酶活性测定表明,给予SRE后,心和肾SOD、CAT、GPx活性比模型组显著提高;造模后肾Mn-SOD表现出代偿性增高,给予SRE后能使其恢复到对照组水平。各脏器CAT同工酶都为1条,在造模后酶带染色密度明显降低,在给予SRE后能恢复到正常水平。肝有1条Mn-SOD带和3条CuZn-SOD带,在给予SRE后能使这些酶蛋白含量达到或高于正常水平;心、肾有1条Mn-SOD带和4条CuZn-SOD带,SRE能增加心、肾CuZn-SOD酶蛋白染色密度。结论:SRE能通过提高抗氧化酶的活性和同工酶酶蛋白含量,有效预防急性肝损伤小鼠肝脏和主要脏器的氧化损伤作用。

Objective：To evaluate the effect of Stropharia rugoso-annulata extract（SRE） on antioxidative enzyme activity and isozyme profile in liver and other major visceral organs of mice with CCl4-induced acute liver injury.Methods：After 4 days of normal feeding,CIR female mice（25 ± 2） g were used as experimental animals.Ten CIR mice orally administered distilled water were served as control group.Fifty-six more CIR mice were randomly divided into 4 groups of 14 mice each：SRE groups orally administered SRE solution in distilled water at low（100 mg/（kg bw·d））,medium（200 mg/（kg bw·d）） and high（400 mg/（kg bw·d）） doses and model group orally administered distilled water.All mice were administered for 30 consecutive days and low-,medium-and high-dose SRE groups and model group were fed water fasting diet for 16 h on the 30th day before oral administration of CCl4 dispersed in salad oil for creating CCl4-induced acute liver injury model.At 24 h postadministration,all mice were sacrificed to assay activities of antioxidant enzymes in liver,heart,kidney and spleen and analyze isozyme profile of CAT and SOD by gradient polyacrylamide gel electrophoresis.Results：Compared with the model group,high-dose SRE group exhibited significantly increased activities of total SOD,Mn-SOD,CuZn-SOD,GPx and CAT in liver;In addition,there was a significant increase of activities of SOD,CAT and GPx in heart and kidney in three SRE groups.Compared with the normal group,the model group exhibited a compensatory increase of Mn-SOD in kidney;however,Mn-SOD activity was decreased to the normal group level after SRE administration.One CAT band was detected in liver,kidney and heart and CCl4-induced acute liver injury resulted in a significant decrease of staining intensity of the brand.In contrast,the staining intensity of the band was increased to the normal group level after SRE administration.Similarly,one Mn-SOD and three CuZn-SOD bands were detected in liver and the staining intensity of the isozymes was also increased to the normal group level after SRE administration.One Mn-SOD and four CuZn-SOD bands were detected in heart and kidney and SRE administration resulted in the increase of staining intensity of CuZn-SOD bands.Conclusion：SRE can protect liver and other major visceral organs of mice with CCl4-induced acute oxidative injury by enhancing the activities of antioxidant enzymes and the contents of isozymes.