西青果提取物对α-葡萄糖苷酶抑制活性的研究(Ⅲ)

in vitro and in vivo Inhibitory Effect of Methanol Extract from Terminalia chebula Retz Fruits on α-Glucosidase

研究西青果提取物对小肠α-葡萄糖苷酶的抑制作用。西青果提取物在体外和小肠模型人类结肠Caco-2细胞中,显示很强的麦芽糖酶抑制活性,而对蔗糖酶的抑制作用不显著。在体内实验中,西青果提取物能降低SD雄性大鼠进食麦芽糖30min后的血糖,而对进食蔗糖后的血糖浓度没有影响。实验结果表明西青果提取物可以预防Ⅱ型糖尿病。

In order to evaluate the inhibitory effect of Terminalia chebula Retz fruit extract（TCFE） on α-glucosidase,Caco2 cells were used to assay the inhibitory activity of TCFE in vitro and in small intestinal model.Results indicated that TCFE exhibited a strong inhibitory effect on maltase,while no obvious inhibitory activity on sucrase in vitro was observed.In addition,TCFE also obviously reduced 30 min postadministration blood glucose level of SD maltose-loaded male rats.On the other hand,this extract had no effect on the postprandial blood glucose level in normal SD rats administrated sucrose.These results suggest that TCFE has a preventive effect against Type Ⅱ diabetes.