氨气熏蒸降解玉米中黄曲霉毒素B_1的条件优化

Optimization of Ammonia Fumigation for Degradation of Aflatoxin B_1 in Maize

为提高氨气对玉米中黄曲霉毒素B1(AFB1)的降解效率,首先通过单因素试验研究氨熏温度、氨气浓度、氨熏时间及玉米含水量对黄曲霉毒素B1降解率的影响,然后通过二次回归正交试验设计及响应面分析确定主要影响因素的最优参数组合:温度37℃、玉米含水量20%、氨气体积分数7.05%、氨熏时间96h,在此条件下玉米中AFB1降解率的模型预测值为90.4%,实测值为92%,两者较接近。

One-factor-at-a-time method and quadratic orthogonal rotational combination design combined with response surface analysis were used to optimize temperature,ammonia concentration,fumigation time and moisture content in maize affecting degradation efficiency of ammonia fumigation towards aflatoxin B1（AFB1） in maize.Results showed that the optimal values of the above parameters were determined as follows：temperature 37 ℃,moisture content in maize 20%,ammonia concentration 7.05% and fumigation time 96 h.Under such conditions,the predicted value of degradation ratio of AFB1 in maize was up to 90.4% and its experimental value was 92%.