响应面法优化野艾蒿总黄酮的超声波提取工艺

Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Artemisia lavandulaefolia Using Response Surface Methodology

为确定野艾蒿中总黄酮超声波提取的最佳工艺条件,以总黄酮得率为指标,采用响应面法对主要工艺参数进行优化并得到回归模型。结果表明:最优工艺条件为超声时间36.7min、提取温度74.99℃、乙醇溶液体积分数71.78%、液固比65.68:1(mL/g),在此工艺条件下的总黄酮得率为2.80%。回归模型的预测值与实测值的相对误差为1.1%,该回归方程与实际情况拟合较好。

Ethanol aqueous solution was used to extract total flavonoids from Artemisia lavandulaefolia under ultrasonic assistance.To optimize extraction yield of total flavonoids,30 experiments resulting from a five-variable and four-level central composite design were first carried out;subsequently,experimental data obtained were subjected to multiple variable regression fitting to establish a mathematical model for extraction yield of total flavonoids as a response to 5 variables and a significance test was performed for the model;and finally response surface analysis was conducted for investigating the interaction effects of 5 variables on extraction yield of total flavonoids.Results showed that the developed model could describe the relationship between extraction yield of total flavonoids and 5 variables such as ultrasonic treatment time,extraction temperature,ethanol concentration in aqueous solution and solid/liquid ratio very well.The optimal values of the variables were as follows：ultrasonic treatment time 36.7 min,extraction temperature 74.99 ℃,ethanol concentration in aqueous solution 71.78%,and solid/liquid ratio 1：65.68（g/mL）.Under such conditions,the predicted value of extraction yield of total flavonoids was 2.8%,which was very consistent with the experimental value with a relative error of 1.1%.