D-101大孔吸附树脂分离纯化橘皮中的黄酮类物质

Purification of Crude Total Flavonoids Extracted from Citrus Peel Using D-101 Macroporous Adsorption Resin

目的:研究D-101大孔吸附树脂分离纯化橘皮黄酮类物质的工艺条件。方法:采用静态和动态吸附-解吸附两种方法,以黄酮类物质吸附率和解吸附率为评价指标,考察橘皮提取液pH值、吸附液料比、静置吸附时间、洗脱液种类和洗脱液料比等影响因素。结果:D-101大孔吸附树脂分离纯化橘皮黄酮类物质的最佳工艺条件为提取液pH4.43,吸附液料比15:1(黄酮类物质溶液:大孔吸附树脂,mL/g)、静置吸附时间90min、洗脱液为95%乙醇溶液、洗脱液料比25:1(95%乙醇溶液:大孔吸附树脂,mL/g)。结论:该方法简单、可行,能够用来分离纯化橘皮中黄酮类物质。

To propose an optimal purification process of total flavonoids in 70% ethanol extract from citrus peel,the static and dynamic adsorption-desorption performance of D-101 macroporous adsorption resin towards total flavonoids from citrus peel was investigated.Major process parameters including pH of sample solution（ethanol extract of citrus peel）,sample solution volume/D-101 resin weight ratio（mL/g）,standing time and kind and amount（desorption solvent volume/D-101 resin weight ratio,mL/g） of desorption solvent affecting either adsorption ratio or desorption ratio of total flavonoids were evaluated and their optimal values were determined to be pH 4.43,15：1,90 min,95% ethanol and 25：1,respectively.The method proved to be simple and feasible for purification of crude total flavonoids extracted from citrus peel.