反相高效液相色谱法快速测定婴儿配方乳粉中的维生素A酯类化合物

Rapid Determination of Retinyl Esters in Infant Formula Milk Powder by Reversed-Phase High-performance Liquid Chromatography

目的:开发一种快速测定婴儿配方乳粉中维生素A酯类含量的反相高效液相色谱法。方法:直接使用二氯甲烷和甲醇体积比为2.5:1的混合溶剂对样品进行提取,在30℃的条件下采用等度洗脱,流动相采用体积比为80:20的甲醇-三氯甲烷溶剂,流动相流速1.0mL/min;色谱柱温度30℃,检测波长325nm。结果:维生素A醋酸酯和维生素A棕榈酸酯的回收率分别为100.1%和102.8%,多次测定结果的相对偏差分别为1.18%和0.96%,检出限分别为3.1ng和4.3ng。结论:本方法操作简单、精密度、回收率和灵敏度高,适用于婴幼儿配方乳粉中的维生素A酯类的检测。

Objective：To develop a rapid reversed-phase high performance liquid chromatographic（RP-HPLC） method for measuring retinyl esters in infant formula milk powder.Methods：Samples were directly extracted with a mixture of dichloromethane and methanol（2.5：1,V/V）.The chromatographic separation was performed at 30 ℃ in the isocratic elution mode using a mobile phase composed of a mixture of methanol and chloroform（80：20,V/V）.Results：Retinyl acetate and retinyl palmitate presented average spike recoveries of 6 replicates at 2 levels of 100.1% and 102.8%,with relative standard deviations of 1.18% and 0.96%,respectively.The detection limits for retinyl acetate and retinyl palmitate were 3.1 ng and 4.3 ng,respectively.Conclusion：The method demonstrates simplicity and high precision,recovery and sensitivity,and thus has promising potential for practical applications.