液质法测定猪肉中十种β-兴奋剂残留

HPLC-MS/MS Determination of Beta-Adrenergic Agonist Residues in Pork

建立猪肉中β-兴奋剂多残留的液相色谱-串联质谱(LC-MS/MS)分析方法。样品经酸性乙腈提取,固相萃取柱净化后采用LC-MS/MS进行检测分析,对样品前处理条件和质谱参数进行研究。结果表明:这些激素的物质的线性范围均为0.5～100μg/kg;低、中、高3种加标水平回收率为72%～87%;最低检出限为0.1～1μg/kg。本方法快速简便、灵敏度高、选择性好,测定结果令人满意,可在各食品检测机构推广应用。

A sensitive and rapid HPLC-MS/MS method was developed for the determination of 10 Beta-adrenergic agonists residues in pork.Samples were subjected to extraction with acidified acetontrile,followed by solid phase extraction clean-up before HPLC-MS/MS analysis.The developed standard curves of 10 Beta-adrenergic agonists all displayed good linearity over the concentration range of 0.5-100μg/kg.Mean recovery rates of 6 replicates for Beta-adrenergic agonist in a negative pork sample spiked at three levels were between 72% and 87%.The limit of detection of the method ranged from 0.1 to 1μg/kg.This method proved to be of simplicity,high sensitivity and good selectivity and provided satisfying determination results.Therefore,its application deserves to be popularized among various food testing laboratories.