GC测定止咳丸中(-)薄荷酮和薄荷脑的含量

Determination of (-)menthone and menthol in Coughpill by GC

目的建立止咳丸中(-)薄荷酮、薄荷脑含量测定方法。方法色谱柱HP-FFAP毛细管柱(25m×0.2mm,0.3μm);程序升温:初始温度90℃,保持2min,以6℃·min-1的速率升至150℃,再以30℃·min-1的速率升温至220℃,保持4min;载气为高纯氮,氢火焰离子化检测器(FID),进样口温度:230℃;检测器温度:250℃;分流比为10:1,进样量1.0μL。结果 (-)薄荷酮在0.04486～0.8972mg·mL-1(r=0.9997)内平均回收率为98.12%,RSD=0.86%;薄荷脑在0.06488～0.9732mg·mL-1(r=0.9998)内平均回收率为100.91%,RSD=1.16%,均呈良好线性关系。结论本法快速、简便、准确、可靠,可用于控制止咳丸的质量。

OBJECTIVE To establish a method for the determination of （-）menthone and menthol in coughpill. METHODS The analysis was carried out on an HP-FFAP capillary column（25 m×0.2 mm, 0.3 μm）. Column temperature was at 90 ℃（2 min）, 6℃min-l→150℃, 30 ℃min -1→220℃（4 min）.Carrier was N2. Detector was FID. Temperature of the column entrance was 230 ℃. Temperature of the detector was 250℃. Fractional flow ratio was 10：1. Sample quantity was 1.0 μL. RESULTS The linear range of （-）menthone was 0.044 86-0.897 2 mg＇mL-l（r=0.999 7）. The average recovery was 98.12%, RSD=0.86%. The linear range of menthol was 0.064 88-0.973 2 mg.mL 1（r=0.9998）. The average recovery was 100.91%, RSD=l.16%. CONCLUSION This method is simple, quick, accurate and reliable for the quality control of coughpill.