火焰原子吸收光谱法测定人血红细胞中锌、铁、镁

Direct Determination of Zinc,Iron,Magnesium in Human Red Blood Cells by FAAS

人血红细胞样品经低渗Triton X-100溶液超声振荡溶血处理并稀释100倍后采用火焰原子吸收光谱法测定其中锌、铁和镁的含量。锌、铁和镁的检出限(3S/N)分别为2.44×10^(-3),2.85×10^(-2),1.56×10^(-3)mg·L^(-1),相对标准偏差(n=7)分别为0.69%～1.03%,2.85%～3.84%,0.92%～1.22%。应用该法分析同一混合人血红细胞样品,测定结果与酸消化法测得的结果一致,3种元素的加标回收率为95.0%～110.0%之间。

The sample of human red blood cell was treated to haemolyze with hypisotonic cold Triton X-100 solution by ultrasonic vibration.The the sample solution was diluted one hundred times,and the contents of Zn,Fe and Mg were directly determined by FAAS.The values of detection limit（3S/N） and RSD＇s（n=7） for Zn,Fe and Mg were 2.44×10^-3,2.85×10^-2,1.56×10^-3 mg·L^-1 and in the ranges of 0.69%-1.03%,2.85%-3.84%, 0.92%-1.22%,respectively;The method was applied to the analysis of a same sample of mixed human red blood cell,and the results obtained were in consistency with those obtained by the acid digestion method.Values of recovery of the 3 elements were in the range of 95.0%-110.0%.