LC-ESI-MS同时测定大鼠血浆中盐酸小檗碱、大黄素、大黄酚和大黄素甲醚的含量

Simutaneously Determination of Berberine Hydrochloride,Emodin,Chrysophanol and Physcion in Rat Plasma after Oral Administration of Sanhuang Tablet by LC-ESI-MS

目的:建立同时测定大鼠血浆中盐酸小檗碱、大黄素、大黄酚和大黄素甲醚含量的方法。方法:采用高效液相色谱串联质谱法,色谱柱为Shim Pack VP-ODS C_(18)色谱柱(150 mm×2.0 mm,5μm),柱温30℃,流动相为乙腈:0.5%甲酸水溶液(80:20,氨水调pH=3.5),流速0.2ml·min^(-1)。结果:盐酸小檗碱、大黄素、大黄酚、大黄素甲醚的最低检测限范围为1.0～1.2ng·ml^(-1),定量下限范围为5.0～11.5 ng·ml^(-1),日内、日间RSD<2.98%。结论:该法准确、快速,可用于上述4种成分的药动学研究。

Objective ： To Establish a LC-ESI-MS method to quantify the concentrations of berberine hydroehloride, emodin, chryr sophanol and physcion in rat plasma after oral administration of Sanhuang tablet. Method： Liquid chromatography coupled with Mass spectrometry, with Shim Pack VP-ODS Cls analytical column （150 mm × 2.0 mm, 5 μm）was used, oven temperature was 30%1 and the mobile phase was a mixture of acetonitrile and 0.5% formic acid solution （ adjust pH to 3.5 with ammonia） , flow rate was 0.2 ml · min^ - 1 . Result ： The limit of determinations （ LODs ） of the three constituents were ranged from 1.0 to 1.2 ng · ml^ - 1 , the lower limit of quantitations （LLOQs） were ranged from 5.0 - 11.5 ng · ml ^-1 , RSDs of intra-day and inter-day precision were less than 5%. Conclusion： The method could be used for the fast and accurate determination of the four components in rat plasma, and it was successfully applied for the investigations of their pharmaeokinetic properties.