罗汉果的质量标准

Study on Quality Standard for Fructus Momordicae

目的:建立测定罗汉果中多糖、总糖及罗汉果苷V含量的方法,并测定其不同部位的含量。方法:采用苯酚-硫酸比色法测定多糖及总糖含量,测定波长为487nm;用眦测定罗汉果苷V含量,PhenomenexC_(18)柱,乙腈-水(22:78)为流动相,流速1.0 ml·min^(-1),检测波长208 nm。结果:无水葡萄糖在32.0～192.2μg,r=0.999 8,罗汉果苷V在0.81～12.1μg范围内具有良好的线性关系(r=0.999 9),平均回收率分别为100.7%和102.0%,RSD为2.33%和1.03%(n=6);其含量均以果肉最高而种子较低。结论:方法灵敏、准确,重复性好,适用于该药材的质量评价。

Objective： To establish a methods for determination the contents of polysaccharides, saccharides and mogroside V in Fructus Momordicae from various sample and part. Method： For poiysaccharides and saccharides, the phenol sulferic acid colorimetric method was developed and some factors were also optimized,the detection wavelength was 487 nm, the mogroside V content of Fruetus Momordicae was determind by HPLC. Result： The calibration curves of glucose anhydrous and mogroside V were linear within the range of 32.0-192.2 pLg （ r = 0. 999 9 ）, 0.81-12.1 p.g （ r = 0.999 9 ）, the average recoveries （ n = 6 ） were 100.7 %, 102.0%. with RSD were 2.33% , 1.03% respectively, their contents were the highest in flesh and peel, Lowerest in seed. Conclusion： The method is sensitive, accurate and repeatable. It can be used for quality contrnl of Fructus Momordicae.