摘要
目的:建立高效液相色谱法分析左旋多巴的含量及其有关物质,并对有关物质进行定位。方法:采用Agilent TC-C_(18)色谱柱(150 mm×4.6 mm,5μm);以0.1%三氟乙酸溶液-乙腈(96:4)为流动相;流速1.0 ml·min^(-1);检测波长为280nm;柱温:室温。结果:左旋多巴和L-酪氨酸分别在0.06～0.61 mg·ml^(-1)和5.01～50.12μg·ml^(-1)范围内线形关系良好,r=0.999 9,各杂质均能达到很好的分离。结论:该方法能有效的用于左旋多巴的定量及有关物质的检测。
Objective： To establish a method for determination of levodopa and its related substances by HPLC. Method： The chromatographic column of Agilent TC - C18 （ 150 mm × 4.6 mm,5 μm ）was used , the mobile phase was 0.1% trifluoroacetic acid - acetonitrile （96：4 ） with flow rate of 1.0 ml · min ^- 1, the detection wavelength was set at 280 nm and the chromatography was operated at room temperature. Result： The calibration curve of levodopa and L -tyrosine was in good linearity over the range of 0.06 -0.61 mg · ml^-1 and 5.01 - 50.12 μg · ml^-1 （ r = 0. 999 9 ） , respectively. Related substances could be separated well. Conclusion： This method is convenient and accurate for the assay of levodopa and also suitable to identify of its related substances.
出处
《中国药师》
CAS
2010年第5期681-683,共3页
China Pharmacist
