摘要
目的:建立HPLC法测定酒石酸美托洛尔注射液的含量和有关物质。方法:色谱柱:phenomenex luna C_(18)柱(150 mm×4.6 mm,5μm),流动相:甲醇-庚烷磺酸钠缓冲溶液(水450ml,加庚烷磺酸钠960 mg,加无水醋酸钠82 mg,加冰醋酸0.57ml)(49:51),流速:1.0 ml·min^(-1),检测波长:275 nm。结果:在选定的色谱条件下,主药与杂质分离良好。酒石酸美托洛尔在28.94～964.80μg·ml^(-1)范围内线性关系良好(r=0.999 9,n=6)。结论:该方法可用于酒石酸美托洛尔注射液的含量和有关物质的测定。
Objective： To estable a method for determination of metoprolol tartrate injection and related substances by HPLC. Method： The phenomenex luna C18 column （150 mm× 4.6 mm ,5 μm） was used, the mobile phase was consisted of methanolsodium heptanesulfonate buffer （ water 450 ml, adds sodium heptanesulfonate 960 mg, anhydrous sodium acetate 82 mg and acetic acid 0.57 ml）（49： 51） ,the flow rate was 1.0 ml · min^-1 and wavelength of detector was at 275 nm. Result： At the selected chromatographic conditions, the main drug was well separated from related materials and determined with a good linear relationship within the range of 28.94-964.80 μg · ml^-1（ r = 0. 999 9, n = 6 ）. Conclusion： The method can be used to determine the metoprolol tartrate and the related substances.
出处
《中国药师》
CAS
2010年第5期693-694,共2页
China Pharmacist
