复肝宁对实验性肝纤维化大鼠血清标志物和肝脏超微结构的影响

Effects of Fuganning on serum indicators and hepatic ultrastructure changes in rats with hepatic fibrosis

目的:观察中药复肝宁对实验性肝纤维化的预防效果,探讨其作用机制。方法:采用猪血清腹腔注射法造成大鼠免疫性肝纤维化模型,观察复肝宁对实验性肝纤维化大鼠血清标志物透明质酸(HA)、层黏连蛋白(LN)、Ⅳ型胶原(CⅣ)含量、肝组织病理学和超微结构的影响。结果:肝纤维化大鼠在应用复肝宁后,血清HA、LN、CⅣ含量明显降低,肝组织病理损害、细胞坏死明明显减轻,胶原纤维明显减少,与模型组比较差异有显著性意义(P<0.05)。结论:复肝宁能有效地防止免疫性肝纤维化大鼠血清HA、LN、CⅣ含量的升高,减轻肝组织病理损害,维持胶原正常代谢,抑制肝纤维化形成,从而对肝纤维化具有一定的预防作用。

Objective：To research the effects and mechanism of Fuganning（FGN） on rats with hepatic fibrosis.Methods：Rat immuno hepatic fibrosis model was induced by intraperitoneal injection of porcine serum,to observe the effect of FGN on HA,LN,CⅣ,the pathological and ultrastructure chages of hepatic tissue.Results：The level of HA,LN,CⅣ decreased,the pathological chages and the collagen fibresis decreased significantly in FGN group,this groups had significanc difference to model group（P〈0.05）.Conclusion：FGN can reduce the level of HA,LN,CⅣ of model rats.It can affect the collagen protein metabolism.Thus FGN prescription had effect in preventing hepatic fibresis.