针刺对2型糖尿病大鼠肝组织胰岛素受体底物2基因表达的调节

Regulations of acupuncture on hepatic tissue IRS2 gene expression in type 2 diabetes rats

目的:探讨肝组织胰岛素受体底物2(IRS2)基因表达与2型糖尿病(T2DM)发病的关系,以及针刺对T2DM大鼠肝组织IRS2mRNA表达的影响。方法:给食源性肥胖大鼠腹腔注射小剂量链脲佐菌素(STZ)(25mg/kg)造模成T2DM大鼠,随机分为针刺组、优降糖组和模型组,处理4周后,用快速血糖仪检测空腹血糖(FBS)、用放免法检测空腹胰岛素(FINS)、用实时定量荧光PCR(real-time RT PCR)法检测肝组织IRS2mRNA表达,并与正常组大鼠进行对照。结果:模型组FBS(15.79±1.87)、FINS(37.20±3.92)明显高于正常组(5.16±0.13,23.57±1.63);针刺组FBS(5.46±0.20)、FINS(20.87±1.80)较模型组明显下降;优降糖组FBS(5.26±0.13)明显下降,接近正常组水平,FINS(28.10±1.86)较模型组明显下降;模型组IRS2 mRNA相对含量是正常组的2.19倍,针刺组为正常组的4.04%,优降糖组为正常组的4.59倍。结论:肝组织IRS2mRNA异常表达可能是2型糖尿病的发病机制之一,针刺可以明显抑制T2DM大鼠肝组织IRS2mRNA的异常表达,改善胰岛素抵抗。

Objective：To study the relationship between hepatic tissue insulin receptor substrate 2（IRS2） gene and type 2 diabetes,and the regulation of acupuncture on IRS2 gene expression of hepatic tissue in type 2 diabetes mellitus（T2DM） rats.Methods：Obese rats due to diet were injected in abdomen with small dose streptozotocin（STZ） to induce T2DM.Then these T2DM rats were stochastically divided into acupuncture group,Glibenclamide group and model group.After 4 weeks treatment,fasting blood sugar（FBS） was examined with active blood sugar meter,fasting insulin（FINS） was examined by radioimmunoassav,hepatic tissue IRS2mRNA was evaluated in real-time RT PCR,and normal rats were in control.Results：Serum levels of FBS（15.79±1.87） and FINS（37.20±3.92） in model group were higher than those in normal group（5.16±0.13,23.57±1.63）,serum levels of FBS（5.46±0.20） and FINS（20.87±1.80） in acupuncture group were obviously lower than those in model group,which were similar to normal group.FBS（5.26±0.13） reduced obviously and serum FINS（28.10±1.86） reduced in some degree in Glibenclamide group compared with model.Relative contents of IRS2mRNA in model group were 2.19 folds compared with that in normal group.And in Glyburide group it was 4.59 folds compared with that in normal group.In acupuncture group it was 4.04% of that in the normal group.Conclusion：Abnormal hepatic tissue IRS2 mRNA expression may be one of pathogenesis of T2DM.Acupuncture can obviously depress hepatic tissue IRS2 mRNA expression,but Glibenclamide can not.