STAT1、STAT6在支气管哮喘小鼠中的表达及地塞米松对其表达的影响

Expression of STAT1, STAT6 in murine bronchial asthma model and effect of dexamethasone on the expression

目的研究信号转导和转录激活因子1（signal transducer and activator of transcription 1，STATl）、信号转导和转录激活因子6（STAT6）在支气管哮喘（简称哮喘）气道炎症中的作用和地塞米松对其表达的干预。方法雄性BALB／c小鼠随机分为对照组（A组）、哮喘组（B组）和地塞米松组（C组），每组8只。逆转录一聚合酶链反应（RT—PCR）法半定量测定肺组织中STATl、STAT6mRNA的表达水平，组织学观察肺组织炎症程度。结果①B组肺组织中STATlmRNA表达水平显著高于A组（P〈O．01），而c组显著低于B组（P〈0．01）；②B组肺组织中STAT6tuRNA表达水平显著高于A组（P〈O．01），而c组显著低于B组（P〈0．01）；③C组小鼠肺组织中STATlmRNA与STAT6mRNA的表达呈显著正相关（r=0．939，P〈O．01）。结论STATl、STAT6的高表达参与了哮喘小鼠的病理生理过程；地塞米松可同时下调STATl、STAT6的表达，抑制哮喘气道炎症。

Objective To investigate the effects of signal transducer and activator of transcription 1 （STAT1）, STAT6 in ovalbumin-challenged asthmatic mice and observe the change of STAT1, STAT6 interfered by dexamethasone. Methods BALB/c male mice were randomly divided into 3 groups： control group,asthma group,and dexamethasone group, with 8 mice in each group. The expressions of STAT1, STAT6 mRNA in lung tissue were detected by RT-PCR. The pathological changes of lung were measured by HE staining. Results Compared with asthmatic group,the expression of STAT1,STAT6 mRNA were lower significantly（ P d0.01） in control group and dexamethasone group. STAT1 mRNA expression of lung tissue in dexamethasone group was correlated with STAT6 mRNA expression significantly （ r = 0. 939, P 〈0.01）. Conclusions The high expression of STAT1 and STAT6 is involved in the patho- physiological process of mouce asthma development. Dexamethasone can down-regulate the expression and inhibit the airway inflammation in asthma.