铅胁迫下转DREB1A高羊茅对铅的吸收与耐受性研究

Research on Lead Uptake and Tolerance of Tall Fescue with Foreign DREB1A under Lead Stress

通过水培试验,研究转DREB1A高羊茅对铅的吸收与耐受性。分别用浓度为100 mg/L、200 mg/L、500 mg/L、1000 mg/L、1500 mg/L、2000 mg/L的铅溶液对生长1个月的高羊茅进行胁迫处理,3周后测算了高羊茅植株的铅含量、枯黄率、根系耐性指数、迁移率、富集系数、转运系数等指标。结果显示,相同的铅胁迫条件下,转基因高羊茅的生长状况较好,对铅的富集与转运能力也较强。但无论是在生长状况方面还是在铅含量及分布方面,两种高羊茅对铅胁迫的反应趋势基本相同。表明DREB1A基因未改变高羊茅对铅的转移运输机制,但提高了高羊茅对铅的富集能力及耐性,转DREB1A高羊茅可成为修复铅污染土壤的备选植物。

In order to find new plant resources for remediation of heavy metal contaminated soil,this experiment,which was carried out under hydroponic culture conditions,was to study lead uptake and tolerance of tall fescue with foreign DREB1A.The materials of this experiment were transgenic and non-transgenic plants.A month later,tested plants were treated with lead stress and the seven sets concentrations of lead were 100mg/L,200mg/L,500mg/L,1000mg/L,1500mg/L,and 2000mg/L.Three weeks later six indexes were measured which were the content and distribution of lead,the withering rate,the index of root tolerance,the translocation rate,bioaccumulation factor and translocation factor.The results showed that the growth satus of the transgenic tall fescue were better than non-transgenic plants at the same condition and it was the same with the enrichment ability and the transport capacity.Whereas whether it was in the growth status or in the content and distribution of lead,two kinds of tall fescue on lead stress has basically the same trend.This showed that DREB1A gene had not changed the tall fescue on the transport mechanism for the transfer of lead,but improved the enrichment ability and the tolerance of tall fescue.All these proved that transgenic tall fescue is fit for phytoremediation of lead.