心肺复苏后大鼠神经元氧自由基的变化及卡尼汀的干预作用

Changes in oxygen free radical of plasma in Rats after Cardiopulmonary Resuscitation and the intervention effect of carnitine

目的:研究心肺复苏后大鼠脑细胞氧自由基的改变及卡尼汀的干预作用。方法:本实验采用窒息合并冰氯化钾停跳液致大鼠心跳骤停5min后开始心肺复苏的动物模型,SD大鼠88只,随机分为11组:对照组(假手术组)、复苏后3、12、24、48、72h组(每组8只),复苏后卡尼汀干预3、12、24、48、72h组(每组8只)。各组抽静脉血测定血液中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活力。结果:心跳骤停/心肺复苏后各组大鼠血清中MDA含量较对照组显著升高(p<0.05),SOD活力显著降低(P<0.05)。使用卡尼汀干预后,血清MDA含量显著降低(P<0.05),SOD活力基本正常(P<0.05)。结论:心肺复苏后大鼠氧自由基产生增多,清除减少;卡尼汀干预后,氧自由基产生减少。卡尼汀对心肺复苏后大鼠具有保护作用。

Objective： To observe Changes in oxygen free radical of plasma in Rats after Cardiopulmonary Resuscitation（CPR）, and to observe the intervention effect of camitine on these changes. Methods： Cardiac arrest was induced by asphyxiation and ice-cold 0.5M KCI and resuscitation was started in five minutes after arrest. Eighty-eight SpragueDawley rats were divided into 11 groups randomly： sham （control group）; after cardiopulmonary resuscitation rats were allowed to reperfuse spontaneously for 3 hours, 12 hours, 24 hours 48 hours and 72 hours （n=8,per group）, after cardiopulmonary resuscitation and earnitine administration rats were allowed to roperfuse spontaneously for 3 hours, 12 hours, 24 hours 48 hours and 72 hours （n=8,per group）. The quantity ofmaleic dialdehyde （MDA） and enzyme activity of superoxide dismutase （SOD） in plasma among all the groups were analyzed by the chromametery;Using the Neural Deficiet Scores（NDS） evaluate the nerve functions of rats. Camitine（100mg/kg） was giving intraperitoneally after Restoration of spontaneous circulation （ROSC）. Results： After CPR, the levels of MDA in plasma were higher than that in the mock rats （p〈0.05）, while the levels of SOD were obviously lower than that in the mock rats （p〈0,05） ,with the administration of LC, The damage can be ameliorated by the administration of LC. Conclusion： The damage of oxygen free radical was resulted by CA/CPR.Cmitine plays an important role in the protection of brain cells in Rats alter CPR.