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流体力学注射IL-35质粒对ConA诱导小鼠肝损伤的保护作用 被引量：2

Protective Effect ofHydrodynamic-Based Delivery of Interleukin-35 Gene on Liver Injury Induced by Concanavalin A in Mice
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摘要 目的:观察流体力学注射白细胞介素35(IL-35)质粒对刀豆蛋白A(ConA)诱导的小鼠肝损伤的干预效果。方法:通过流体力学注射将pcDNA3.1-IL-35导入C57BL/6小鼠体内,对照组注射pcDNA3.1。24h后,以ConA(15mg/kg)尾静脉注射建立小鼠肝损伤模型,检测血清ALT活性和肝组织病理变化。结果:流体力学注射pcDNA3.1-IL-3524h后,肝组织有IL-35mRNA和蛋白的表达。与对照组相比,pcDNA3.1-IL-35预处理组小鼠血清ALT水平明显降低,肝组织病理损伤明显减轻。结论:流体力学注射IL-35质粒对ConA诱导的小鼠肝损伤具有保护作用。 Objective： To study the effect of hydrodynamics-based delivery of IL-35 gene on liver injury induced by Concanavalin A in mice. Methods： The murine model was established by intravenous injection of 15 mg/kg Con A, IL-35-treated mice were injected with pcDNA3.1-IL-35 and control mice with pcDNA3.1 using hydrodynamics-based gene delivery 24 hours prior con A injection. The degree of liver injury was assessed by serum transaminase analysis and hepatic histopathology. Results： The expression of IL-35 mRNA and protein in the liver could be detected at 24 hours after hydrodynamic injection of pcDNA3.1-IL-35. Pretreatment with pcDNA3. 1-IL-35 significantly decreased the level of serum ALT and the severity of liver damage compared with mice pretreated with pcDNA3.1. Conclusion： The results indicate that hydrodynamics-based delivery of IL-35 gene can protect against Con A induced liver injury and suggest a novel way for treating human liver disease.
作者 周艳春 李明才 牛青霞 柳晓金 谢燕飞
机构地区 汕头大学医学院炎症与免疫性疾病研究所
出处 《现代生物医学进展》 CAS 2010年第6期1097-1099,1068,共4页 Progress in Modern Biomedicine
关键词 白细胞介素35 刀豆蛋白A 肝损伤 流体力学注射 Interleukin-35 Con A Liver injury Hydrodynamic-based gene delivery
分类号 Q95-3 [生物学—动物学] R392.11 [医药卫生—免疫学]
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参考文献10

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二级参考文献13

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共引文献1

同被引文献30

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现代生物医学进展
2010年 第6期

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