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Ⅰ型单纯疱疹病毒感染的细胞培养系统的建立 被引量：1

Establishment of HSV-1 Infected Cell Culture System
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摘要 目的:建立稳定的HSV-1感染的细胞培养系统,为HSV-1感染性皮肤病的基础及临床研究提供稳定的平台。方法:以猴肾细胞(Vero)为繁殖细胞,选择人鼻咽癌上皮细胞(Hep-2)为靶细胞,观察HSV-1在Hep-2株中的致细胞病变效应(CPE)。结果:HSV-1在Vero细胞中能稳定、大量地繁殖;HSV-1感染Hep-2细胞后可出现明显的细胞病变;结论:以Vero为繁殖细胞,Hep-2为靶细胞的稳定的细胞培养系统可作为HSV-1感染性皮肤病的基础及临床研究的平台。 Objective：To establish a stable HSV-1 infected cell culture system,in order to provide a stable platform for further basic and clinical research of skin disease caused by HSV-1 .Methods：HSV-1 strains were propagated in vero cells and hep-2 cells were served as targets of HSV-1 infection.Then cytopathic effect （CPE） was performed and observed. Results：Abundance of HSV-1 were stable propagated in Vero cells; The CPE occurred obviously in hep-2 cells after infected by HSV-1. Conclusion：The HSV-1 infected cell culture system we established was stable for utilization,in which HSV-1 strains were propagated in vero cells and hep-2 cells were served as targets of HSV-1 infection. The system could be used as a stable platform for further basic and clinical research of skin disease caused by HSV-1.
作者 梁义 林有坤 梁铭
机构地区 柳州市柳铁中心医院皮肤科 广西医科大学第一附属医院皮肤科
出处 《现代生物医学进展》 CAS 2010年第5期858-860,共3页 Progress in Modern Biomedicine
基金 广西自然科学基金资助(桂科0135036)
关键词 单纯疱疹病毒Ⅰ型 细胞培养 猴肾细胞 人鼻咽癌上皮细胞 HSV-1 Cell Culture Vero Hep-2
分类号 R373 [医药卫生—病原生物学] Q75 [生物学—分子生物学]
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参考文献11

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二级参考文献14

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现代生物医学进展
2010年 第5期

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