摘要
采用PCR方法,从全雌系黄瓜基因组DNA中扩增出2333bp的基因片段,BLAST分析结果表明:所克隆的基因序列与Cs-ACS1仅有1个碱基的差异,与Cs-ACS1G的核苷酸序列同源性为98%,而推导氨基酸完全一致;结果表明所克隆的基因为Cs-ACS1基因.构建了该基因的正义表达载体,并导入黄瓜,获得2株转基因植株.
A 2333-bp fragment was amplified from cucumber genomic DNA by means of PCR.BLAST analysis showed that the cloned fragment shared 98% homology with Cs-ACS1G,only one base being different from the recorded sequence,and no change was caused in the deduced amino acid sequence.A sense expression vector was constructed and transformed into cucumber,and two transgenic cucumber plants were obtained.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期79-82,共4页
Journal of Southwest University(Natural Science Edition)
基金
重庆市自然科学基金资助项目(CSTC
2008BB1255)
