姜黄素对心力衰竭兔肌浆网钙泵表达的影响

Effects of curcumin on sarcoplasmic reticulum Ca^2＋ -ATPase in rabbits with heart failure

目的探讨姜黄素对心力衰竭（心衰）兔肌浆网钙泵表达的影响。方法采用主动脉瓣反流联合腹主动脉缩窄制作慢性心衰家兔模型。随机分为心衰姜黄素组、心衰安慰剂组、对照姜黄素组、对照安慰剂组。8周后计算心脏重量与体重比值，观察超微结构，检测肌浆网钙泵mRNA和蛋白的表达水平及活性。结果心衰姜黄素组和心衰安慰剂组心脏重量与体重比值均大于对照组（P〈0．05）；且心衰姜黄素组比值小于心衰安慰剂组（P〈0．05）。电子显微镜显示心衰姜黄素组的心脏超微结构有所改善。心衰姜黄素组和心衰安慰剂组肌浆网钙泵mRNA、蛋白表达及活性均小于对照组（P〈0．05），但心衰姜黄素组均显著高于心衰安慰剂组（P〈0．05）。结论姜黄素能在mRNA水平和蛋白水平提高心衰家兔肌浆网钙泵的表达，提高肌浆网钙泵的活性，这可能是姜黄素改善心衰的机制之一。

Objective To investigate the effects of curcumin on sarcoplasmie retieulum Ca^2＋- ATPase in heart failure rabbits. Methods Rabbit heart failure model was made with aortic regurgitation and abdominal aorta constriction and 40 rabbits were randomly divided into 4 groups including ： （ 1 ） heart failure treated with cureumin; （2） heart failure treated with placebo; （3） healthy control treated with curcumin and （4） healthy control treated with placebo. All rabbits were administrated with eureumin capsules or placebo capsules 100 mg·kg^-1 · d^-1, respectively. All groups were sacrificed after eight weeks. Myocardial uhrastructural organization was detected by transmission electron microscope. RT-PCR and Western blot were used to measure the expression of sarcoplasmic reticulum Ca^2＋ -ATPase in mRNA and protein levels, respectively. Malachite green colorimetric assay was used to evaluate the activity of sareopiasmic retieulum Ca^2＋-ATPase. Results All detected parameters were similar between control curcumin group and control placebo group. Compared with the control groups （ Groups 3 and 4 ） , the heart/body weight ratio was significantly increased in the heart failure-curcumin group （ Group 1 ） and the heart failure-placebo group （ Group 2, all P 〈 0.05 ） , but the ratio was significantly lower in heart failure-curcumin group than in heart failure-placebo group （ P 〈 0.05 ）. The degree of heart failure was decreased by curcumin. Activity and mRNA and protein expression for sarcoplasmic reticulum Ca^2＋ -ATPase were significantly reduced in the heart failure-placebo group and which could be significantly attenuated by curcumin （ all P 〈 0. 05 ）. Conclusion Curcumin could improve cardiac function via upregulating the expression of sarcoplasmic reticulum Ca^2＋ -ATPas in this model.