北疆地区辣椒上烟草花叶病毒(TMV)各分离物的差异性研究

Differenation of the Isolates of Tobacco Mosaic Vrius from Pepper in North Xinjiang

以ＴＭＶ－ｏｍ为对照，测定了辣椒上５个烟草花叶病毒分离物Ｐ－９、Ｐ－３８、Ｐ－４４、Ｐ－７１、ＴＭＶ－ｗｙｍ在４２个辣椒品种、品系上的致病反应，结果表明这５个分离物对辣椒的致病性存在明显差异：Ｐ－７１、ＴＭＶ－ｗｙｍ＞Ｐ－９＞Ｐ－３８、Ｐ－４４；对接种过５个ＴＭＶ分离物的辣椒（四平头）进行过氧化物酶（ＰＯＤ）和酯酶（ＥＳＴ）同工酶分析，结果表明辣椒上５个ＴＭＶ分离物引起的酶活性有差异：导致ＰＯＤ上活性强弱为ＴＭＶ－ｗｙｍ＞Ｐ－９、Ｐ－３８＞Ｐ－４４、Ｐ－７１；引起ＥＳＴ活性强弱为Ｐ－３８＞ＴＭＶ－ｗｙｍ、Ｐ－４４＞Ｐ－７１＞Ｐ－９。说明北疆地区辣椒上ＴＭＶ存在着分化。

Pathogenic reaction of 5 isolates of tobacco mosaic virus from pepper on 42 pepper varieties (cultivars) were tested using TMV om as control.It is shown that pathogenicity of the TMV isolates exists distinct differentiation:P 71、TMV wym>P 9>P 38、P 44;Pepper(si ping tou) inoculated with 5 isolates were analyzed by polyacrylamide gel electrophoresis of the peroxidase(POD) isozymes and esterase(EST) isozymes.It is shown that activity of isozymes caused by isolates have differentiation:activity of POD is TMV wym>P 9、P 38>P 44、P 71,and activity of EST is P 38>TMV wym、P 44>P 71>P 9。Thus it can be seen that TMV from pepper in North Xinjiang exist differentiation。