摘要
目的探讨正电子成像技术应用于阿尔茨海默病（AD）大鼠模型验证的可行性。方法建立Aβ1-40海马注射模型，进行Moms水迷宫测试、HE染色及刚果红染色，观察大鼠行为学、组织学改变。应用PET及MicroPET活体显像显示大鼠脑内2-（4’-N．11C-甲胺基苯）-6-羟基苯并噻唑（N．-11CH3-6-OH-BTA-1，简称11C-PIB）及18F-氟代脱氧葡萄糖（18F-fluordeoxyglucose，简称18F-FDG）的摄取及分布，与行为学、组织学进行比较。结果18F-FDG显像中模型组大鼠海马区域放射性摄取明显低于正常组（P〈0．01），与学习记忆能力减退、神经元丢失相符；11C-PIB显像中模型组大鼠脑内注射区域PIB特异性结合β-淀粉样蛋白，放射性摄取明显高于正常组，与病理改变淀粉样蛋白沉积相符。结论正电子成像技术利用11C-PIB及18F-FDG两种核素联合显像，能够在体显示AD大鼠脑内的斑块沉积及海马区域糖代谢改变，可用于活体验证老年痴呆模型的建立。
Objective To discuss the value of nC-PIB PET and lSF-FDG micro PET imaging in Alzheimer＇s disease（ AD） rat model. Methods The AD rat model was established by injection of Aβ-40 into rat＇s hippocampus. Learning and memory function were estimated by the Morris water maze. Amyloid deposit and neuron loss were observed by Congo red staining and HE staining respectively, nC-PIB PET and 18 F-FDG micro PET scan were performed. Meanwhile the findings of PET imaging were compared with the results of behavior test and histology. Results The uptake of 1SF-FDG in hippocampus of AD rat model group was lower than that of control group（P 〈0. 01 ）. The Morris water maze showed that the escape latent period of rat model group was longer than that of control group （ P 〈 0. 01 ）. Neuron loss was found in rat model brain. Thus the result of micro-PET imaging was matched with those of the Morris water maze and HE staining, 11c-PIB was specifically bound to β-amyloid in rat model brain, the uptake of UC-PIB in AD rat model was higher than that of control （ P 〈 0. 05 ）. Aβ deposit was observed in Congo red staining so that the result of 1, C-PIB Micrp-PET imaging was matched with that of 13-amyloid deposition. Conclusion 11C-PIB Micrp-PET imaging shows the plaque deposition while 18F-FDG -PET imaging reflects the change of glucose metabolism in hippocampus in AD rat model. And PET scan can be used to verify the successful establishment of AD model rat model.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2010年第13期924-927,共4页
National Medical Journal of China
基金
基金项目：国家“863”计划重大专项（2002AA205081）
