超声造影定量评价兔肾缺血再灌注损伤

Quantitative evaluation of rabbit renal ischemia reperfusion injury by contrastenhanced ultrasound

目的通过灰阶超声造影及其量化分析技术评价兔肾缺血后不同时间的再灌注损伤。方法术前先对10只新西兰家兔行左肾超声造影检查作为对照(R_0组,n=10);再将10只家兔随机分为术后缺血30 min后再灌注30 min组(R_1组,n=5)和再灌注60 min组(R_2组,n=5),分别建立肾缺血再灌注模型。灰阶超声造影观察各组肾皮质灌注,并分析灌注峰值时间(TP)、灌注峰值强度(A)、Qontraxt时间-强度曲线(TIC)上升斜率(β)和曲线下面积(AUC)。结果随着再灌注损伤加重,超声造影显示肾皮质灌注回声无明显变化;TP和AUC呈增加趋势,β值逐渐降低,组间差异有统计学意义(P<0.05或P<0.01),其中AUC变化显著(P<0.01);A值无明显变化(P>0.05)。结论 AUC是评价兔肾缺血再灌注损伤的有效指标。

Objective To evaluate rabbit renal ischemia reperfusion injury （ IRI） by gray-scale contrast-enhanced ultrasonography （ CEUS） and quantitative analysis techniques. Methods Ten New Zealand rabbits were performed left renal ultrasonography before surgery and were served as controls （ R_0 group, n = 10）, and then were randomly divided into R_1 group （ reperfusion for 30 min after ischemia for 30 min group, n = 5） and R_2 group （ reperfusion for 60 min after ischemia for 30 min group, n = 5） to establish the models of renal IRI. The pattern of renal perfusion was observed by CEUS, and time to peak （TP）, amplitude of peak intensity（ A）, rising slope （ β） of Qontraxt time-intensity curve and area under the curve （AUC） were analysed. Results With the increase of reperfusion injury, there was no significant change in renal cortex echo revealed by CEUS. TP and AUC increased, β values decreased （P 〈 0. 05 or P 〈 0. 01）, and the change of AUC was even more significant （ P 〈 0. 01）. Besides, there was no significant change in A values among groups （ P 〉 0.05）. Conclusion AUC is an effective index for evaluating rabbit renal IRI.