转录因子E2F-4在中低位直肠癌组织中的表达及意义

Expression of E2F-4 in middle or lower rectal cancer and its clinical significance

目的检测中低位直肠癌组织中转录因子E2F-4的表达,并分析其与临床病理学参数和预后的关系。方法选取临床资料与肿瘤病理标本完整的60例中低位直肠癌患者,所有患者均行直肠癌根治术,另以肠镜活检30例慢性结肠炎的直肠黏膜组织作为对照。免疫组织化学法检测直肠癌组织、癌旁组织及对照直肠黏膜的E2F-4表达,分析其与患者性别、年龄、肿瘤大小、肿瘤分化程度、淋巴结转移、TNM分期和术后转移复发等临床病理参数以及预后的关系。结果直肠癌组织E2F-4表达显著高于癌旁组织和对照直肠黏膜(P<0.05);直肠癌组织E2F-4表达与肿瘤分化程度、TNM分期、淋巴结转移和术后转移复发有关;直肠癌组织E2F-4表达阴性者术后平均无瘤生存时间显著大于阳性者(P<0.05)。结论转录因子E2F-4与直肠癌生物学行为密切相关,在中低位直肠癌的发生发展中可能发挥癌基因的作用。

Objective To detect the expression of transcription factor E2F-4 in middle or lower rectal cancer, and investigate its relationship with clinicopathological parameters and prognosis. Methods Sixty specimens of middle or lower rectal cancer were collected with complete clinical and pathological data, and all the patients were performed radical resection of rectal cancer. Besides, rectal mucosa of 30 patients with chronic colitis examined by enteroscopic biopsy were served as controls. The expression of E2F-4 of rectal cancer tissues, adjacent noncancerous tissues and control rectal mucosa was detected by immunohistochemical method, and its relationship with prognosis and clinicopathological parameters such as gender, age, tumor size, tumor differentiation, lymph node metastasis, TNM staging, post-operative metastasis and recurrence was explored. Results The expression of E2F-4 in rectal cancer tissues was significantly higher than that in adjacent noncancerous tissues and control rectal mucosa （P 〈0.05）. The expression of E2F-4 in rectal cancer tissues was significantly related to tumor differentiation, TNM staging, lymph node metastasis and post-operative metastasis and recurrence. The post-operative tumor-free survival time in patients with negative expression of E2F-4 in rectal cancer tissues was significantly longer than that in patients with positive expression of E2F-4 in rectal cancer tissues （P 〈0.05）. Conclusion The expression of transcription factor E2F-4 is closely related to the biological behavior of rectal cancer, and E2F-4 may be involved in the carcinogenesis and development of middle or lower rectal cancer as oncogene.