摘要
土壤中筛选获得一株产碱性蛋白酶的芽孢杆菌。该蛋白酶发酵16h达到产酶高峰，蛋白酶热稳定性较好，50℃条件下处理50min，酶活性残留86％，最适pH值7．7，丙酮、PEG等可有效沉淀蛋白酶，（NH4）2SO4在5℃，pH9．0条件下可沉淀分离蛋白酶，回收率达95％。
A strain Bacillus sp.,of antioxidant alkaline protease,was screened.Its enzymatic activity is maximum after 16h fermentation.The enzyme was stable at 50℃ for 50min,retaining more than 86% of its activity.Optimum pH for enzyme activity was pH7.7.Protease was effectively separated by acetone,PEG.The specific activity was 95% yield when treated by (NH4)2SO4 at pH9.0 and 5℃.
