难治性癫痫患者脑组织中SCG10的表达及其意义

Up-Regulation of Superior Cervical Ganglia Neural-Specific 10 Protein in the Brain of Patients with Intractable Epilepsy

目的了解难治性癫痫患者脑组织中SCG10和MAPK的表达,探讨其在难治性癫痫发生、发展中的作用。方法按随机化原则,在我们建立的难治性癫痫患者术后脑组织库中抽取36例患者的脑组织,用免疫组织化学分别检测SCG10和MAPK的表达,与16例对照组进行比较。并采用免疫荧光双标的方法,检测SCG10和MAPK在病例组中的表达情况。结果免疫组化检测到SCG10在实验组表达(0.4674±0.0258),高于对照(0.3405±0.0207),两组比较有显著性差异(P<0.05);MAPK在实验组表达(0.4217±0.0141),同样也高于对照组(0.3189±0.1422,P<0.05)。免疫荧光双标法,显示SCG10和MAPK表达在同一细胞上。讨论 SCG10与MAPK在难治性癫痫患者的脑组织中表达增加,以及它们的共同表达,提示两者的相互作用可能是难治性癫痫发生发展中的一个重要因素。

Objective To investigate the expression of SCG10 （Superior cervical ganglia neural-specific 10） and MAPK（ Mitogen Actived Protein Kinase） in brain tissue of patients with intractable epilepsy so as to explore the possible roles of them in the pathogenesis of intractable epilepsy. Methods The brain tissues of intractable epilepsy patients （n = 36） was collected. Expression of SCG10 and MAPK were detected by immunohistochemistry, all results were compared with normal control , and we investigated the relationship between SCG10 and MAPK by double-label immunofluorescence staining in the IE group. Result The SCG10 staining intensity of immunohistochemistry in the intractable epileptic tissue was （ 0. 4674 ± 0. 0258 ） was significantly higher than those in the control group （0. 3405 ± 0. 0207, P 〈 0. 05 ）, the express- tion of MAPK （0. 4217 ±0. 0141 ） was also higher than those in the control group （0. 3189 ±0. 1422,P 〈0. 05）. And by double-label im- munofluorescence staining,we investigated the expression of SCG10 and MAPK occurred in the same neuron. Conclusion The results indicate that SCG10 and MAPK overexpression and co-expressionn , the interaction of them may play an important role in the epileptogenesis of intractable epilepsy.