抗H9亚型流感病毒HA抗血清对小鼠的被动免疫预防和治疗研究

Prophylaxis and Treatment of H9 Infection in Mice with Anti-H9 HA Serum

H9亚型流感病毒是引起大流感的潜在威胁.抗血清被动免疫是一种有效的应对流感的方法.采用H9N2禽流感病毒血凝素(hemagglutinin,HA)DNA重组质粒免疫BALB/c小鼠制备抗血清.在致死量同源病毒感染前或后通过尾静脉给小鼠注射不同剂量抗血清,观察小鼠14d内的体重丢失率和死亡率.结果显示,1280血凝抑制单位(hemagglutination Inhibition unit,HIU)抗血清可给小鼠提供至少长达11d的100%预防和1d的80%治疗保护.HA DNA疫苗免疫制备的抗血清可以有效地抵抗同源H9病毒的致死攻击,在H9病毒感染的预防和治疗中发挥作用.

Influenza H9 subtype virus is a potential threat as it can cause pandemic influenza. Passive immunization with antiserum is an effective way against influenza. Antiserum was produced by immunization of BALB/c mice with H9N2 HA recombinant plasmid. Mice interventrally （i. v.） received different doses of antiserum at different days prior to-or post-lethal challenge with homologous virus. Efficacy was determined by observation of survival of mice as well as weight loss. Results showed that 1 280 HIU dose of antiserum can provide mice at least l 1-days-long 100% prophylactic and 1-day-long 80% therapeutic protection. Antiserum generated from immunization of mice with effectively against homologous avian virus infection, recombinant HA-DNA plasmid can protect mice which means it is a potential utilization in the prophylaxis and treatment of H9 avian influenza.