啤酒废水培养复合型生物絮凝剂产生菌的研究

STUDY ON THE CULTURE OF COMPLEX BIOFLOCCULANT PRODUCING BACTERIUMAND INBREWERY WASTEWATER

从空气和活性污泥中筛选出絮凝剂产生菌,对其进行筛选并纯化,得到絮凝活性较高且稳定的菌株M1和M2。以啤酒废水为廉价培养基,对这2株菌的复合菌(M1-2)进行培养,考察各种因素对复合菌絮凝效果的影响。结果表明,M1-2的最佳培养条件为:直接利用啤酒废水,不另外添加碳源和氮源,只需添加质量分数0.5%的KH2PO4;温度30℃,培养基初始pH=8.5,培养时间48 h,摇床转速为160 r.min-1。在此条件下所产生的絮凝剂对质量浓度为50 g.L-1的高岭土悬液絮凝率可达95.0%,说明啤酒废水中含有丰富的营养物质,作为培养基为絮凝剂产生菌M1-2种提供丰富的碳源和氮源,从而可降低培养成本。

Flocculant-producing bacteria was isolated mainly from the air and activated sludge, and strain M1 and M2 which exhibited excellent flocculation activity and stable were obtained by means of screening and purification. Brewage wastewater was used as low-cost medium to culture mixed bacterium M 1-2. The influence of factors of external carbon source, nitrogen source, medium pH value, culture time etc. on strain flocculation effect was investigated and its culture conditions were optimized. The flocculating rate of kaolin suspension was up to 95.0% under the conditions as that the carbon and nitrogen sources was not needed to add, cultivating temperature 30 ℃, initial pH of culture medium 8.5, cultivating time 48 h and shaking speed 160 r ·min ^-1, which shows that the brewery wastewater contained rich nutrient which can provide abundant carbon and nitrogen sources for flocculant- producing bacteria species M 1-2 and it is feasible to take advantage of distillery wastewater to culture the mixed M1-2, so that the culture cost could be reduced greatly.