HPLC测定两种中国蜂胶醇提物化学组成

Analyses on Chemical Compositions of Ethanolic Extractives of Propolis Collected from Two Regions in China by HPLC

建立了同时测定蜂胶醇提物(EEP)中23种多酚类化合物的高效液相色谱(HPLC)分析方法,并用该法测定了分别采自山东济宁和云南西双版纳的EEP。采用ZORBAX Eclipse XDB C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.1%甲酸水溶液,梯度洗脱,流速1.0 mL/min,检测波长256和280 nm,进样量20μL,柱温35℃。结果表明,所用的分离条件可以使混合对照品及EEP均实现良好分离。通过对比保留时间和紫外吸收光谱,从山东蜂胶中鉴定出20种化合物,云南蜂胶中鉴定出14种化合物。两地EEP的HPLC图谱相似度分别为0.417(256 nm)和0.499(280 nm),两者化学成分差异巨大。山东济宁和云南西双版纳分别地处温带和热带地区,不同的气候及植被可能是这种差异的主要原因。

The method of high-performance liquid chromatography （HPLC） was developed to determine 23 polyphenolic compo- nents in propolis. The ethanolic extractive of propolis （EEP） from Shandong（SD） and Yunnan（YN） Provinces respectively were analyzed. The separation was performed on ZORBAX Eclipse XDB C18 column （4.6 ram × 150 ram, 5 μm） by gradient elution. The mobile phase consisted of methanol with 0.1% formic acid aqueous solution at the flow rate of 1.0 mL/min. The detection wavelengths were 256 and 280 nm. The injection volume was 20 μL, and the column temperature was maintained at 35 ℃. The result shows that mixed reference materials and EEPs could be separated well. By comparing the retention time and ultraviolet absorption spectrum, twenty compounds were detected in EEP of Shandong, and fourteen compounds were detected in EEP of Yunnan. Similarity of spectra between extractives of Shandong and Yunnan was 0.417 and 0.499 at 256 and 280 nm, respectively. The chemical compositions of perpolis from Shandong and Yunnan were significantly different. The differences of climate and vege- tation may be the main reasons for the difference, since Shandong and Yunnan locate at temperate and tropic zones respectively.