纤维素酶解预处理法提取杜仲胶的工艺研究

Study on Extracting Eucommia ulmoides Oliv.Gum by Cellulase Pretreatment

以蒽酮比色法测定的酶解液中的总糖含量和酶解杜仲胶的提取率为考察指标,优化纤维素酶解预处理杜仲粕壳的工艺条件。结果表明,纤维素酶预处理杜仲粕壳的最佳工艺条件:温度50℃,pH值3.8,酶的用量为0.30 g,料液比为1∶15(g∶mL),酶解2次,每次3 h。经最佳酶解工艺条件预处理后的杜仲粕壳,第一次提取酶解物中杜仲胶得率达到57.7%,是非酶解物中的1.3倍。采用薄层层析法检测,结果表明经酶解预处理后所提取的胶与非酶解而提取的胶成分基本相同。

The conditions of enzymatic pretreatment of shell of Eucommia ulmoides Oliv. were investigated, using the amount of total sugars from enzymatic hydrolysate of shell of E. ulmoides with anthrone colorimetry and extraction yield of E. ulmoides gum as assessing index. The optimum enzymatic pretreatment conditions were ： temperature 50℃, pH value 3.8, cellulase dose 0.3 g, solid-liquid ratio 1 ： 15 （g： mL）, extracting twice and 3 h each time. E. ulmoides gum was extracted from raw materials pretreated. The gum yield of the first extraction from the pretreated shell was 57.7 %, which was 1.3 times of that from shells without pretreatment. The results of TLC determination showed that the composition of E. ulmoides gum extracted from pretreated shell is basically similar to that from non-pretreated shell.