导数同步荧光法同时测定1-萘胺和2-萘胺

Simultaneous Determination of 1-Naphylamine and 2-Naphylamine by Derivative Synchronous Fluorescent Method

为同时测定2种萘胺异构体的含量,研究了1-萘胺和2-萘胺的同步荧光光谱及其一阶导数同步荧光光谱,利用零交点法避免了它们之间的干扰。在pH=7.5的KH2PO4-NaOH缓冲溶液中,波长差为120nm的条件下,测定了1-萘胺和2-萘胺的同步荧光。进一步对2种萘胺的同步光谱做一阶导数处理,分别在1-萘胺和2-萘胺的导数同步荧光光谱为零的259和290nm处读取另一种异构体的信号值。该值与浓度呈线性关系,线性范围均在4.0×10-7～2.0×10-5mol/L;1-萘胺和2-萘胺的检出限分别是4.0×10-8和2.9×10-8mol/L;RSD均在5%以下。该方法用于产品中2种萘胺的同时测定,获得满意结果。

A fluorescent method for the synchronous determination of 1-naphylamine and 2-naphylamine was developed.In order to avoid the interference from the two isomers,the synchronous fluorescence and first-order-derivative synchronous fluorescence spectra of the two isomers of naphylamine were studied,and the zero-crossing method for reading spectral intensities was adopted.At pH=7.8（phosphate buffered water solution） and Δλ=120 nm,the synchronous fluorescence peaks were at 323 nm and 290 nm for 1-napylamine and 2-naphylamine,respectively.The first order derivative synchronous fluorescence intensities varied linearly with 1-naphylamine and 2-naphylamine concentrations in a range of 4.0×10-7~2.0×10-5 mol/L.The relative standard deviations（RSDs） of them were all below 5%.The limits of detection were 4.0×10-8 and 2.9×10-8 mol/L for 1-naphylamine and 2-naphylamine,respectively.The method was applied to the simultaneous determination of the two naphylamine isomers with satisfactory results.