脑忆源胶囊中淫羊藿苷含量测定

Determination of icariin in Naoyiyuan capsules

目的建立HPLC法测定保健食品脑忆源胶囊中淫羊藿苷含量的方法。方法采用色谱柱：luna C18（150 mm×4.6 mm,5μm）,流动相：乙腈-水（体积比30∶70）,流速：1.0 mL·min^-1,检测波长：270 nm。结果淫羊藿苷在0.069-0.483μg范围内与峰面积线性关系良好,r=0.999 9,平均回收率为95.39%,RSD为2.5%（n=5）。结论所建立的含量测定方法简便准确,可用于脑忆源胶囊的质量控制。

Objective To determine icariin in Naoyiyuan capsules by HPLC.Methods HPLC was performed on Luna C18column（150 mm×4.6 mm,5 μm）with the mobile phase consisted of acetonitrile-water（30∶70）at a flow rate of 1.0 mL·min^-1,and the detection wavelength was at 270 nm.Results Good linearity resulted in the concentration range of 0.069-0.483 μg,r=0.999 9.The average recovery was 95.39% with RSD of 2.5%（n=5）.Conclusion This method is simple and accurate,and can be used for quality control of Naoyiyuan Capsules.