摘要
目的研究大鼠灌胃给药海南牛耳枫提取物后槲皮素在大鼠体内的药物动力学特征。方法采用LC-MS/MS法,测定灌胃给药后大鼠血浆中槲皮素的浓度变化,用DAS2.0药动学软件处理,计算药动学参数。结果大鼠灌胃给药海南牛耳枫提取物后槲皮素在大鼠体内动力学参数为Tmax=(0.195±0.155)h,Cmax=(35.00±15.30)ng/ml,AUC0-t=(66.82±21.77)ng/(ml·h),t1/2=(5.736±2.513)h。结论该法选择性强、灵敏度高,适用于药材及制剂中槲皮素的体内药动学研究。
Objective To studythepharmacokineticcharacteristicof quercetininrat after oral administration of Daphniphullum calucinum Benth extract.Methods The concentrations of quercetin in rats were determined by LC-MS/MS after drug administration and the pharmacokinetic parameters of quercetin were estimated by DAS2.0 computer program.Result The pharmacokinetic parameters were as follows:Tmax =(0.195 ±0.155) h,Cmax =(35.00 ± 15.30) ng/ml,AUC0-t =(66.82 ±21.77) ng/(ml·h),t1/2 =(5.736 ± 2.513) h.Conclusion The method was proved to be specific,sensitive,and suitable for the pharmacokinetic study of quercetin in traditional Chinese medicines and its preparations.
出处
《海南医学》
CAS
2010年第9期24-26,共3页
Hainan Medical Journal
基金
海南省科技计划项目(编号:061006)
海南省教育厅高校科学研究资助项目(编号:Hjkj200753)
2007国家自然科学基金资助项目(编号:NO.30760298)