狼疮肾炎患者肾小管间质病变与肥大细胞巨噬细胞转化生长因子β_1和T细胞表达的相关分析

The correlation between expression of mast cells,macrophage,T-cell,transforming growth factor β_1 and tubulointerstitial lesion in lupus nephritis

目的探讨狼疮肾炎(LN)患者肾组织中肥大细胞(MC)、巨噬细胞、T细胞(CD4+,CD8+)的和转化生长因子(TGF)-β1与LN肾小管间质病变程度的关系。方法收集51例LN患者的肾活检标本,采用免疫组织化学技术检测MC、巨噬细胞、T细胞(CD4+,CD8+)的及TGF-β1在LN患者肾组织中的表达,并分析其与LN肾小管间质病变的关系。结果肾小管间质中浸润的CD4+细胞、CD8+细胞和CD68+细胞数量与对照相比差异均有统计学意义(P<0.05)。MC的表达与LN肾组织间质纤维化程度存在正相关关系(P<0.05);TGF-β1的表达及T细胞(CD4+,CD8+)、CD68+的巨噬细胞浸润与LN肾组织间质炎症存在正相关关系(P<0.05)。结论MC可能是LN肾间质纤维化的重要参与者;TGF-β1可能是MC、巨噬细胞和T细胞的重要趋化因子。

Objective To detect the expression of mast cells （MC）, macrophage （MΦ）, T-cell, transforming growth factor （TGF）-β1 in kidneys of patients with lupus nephritis （LN）, and to explore its correlation with the tubulointerstitial lesion （TIL） in LN. Methods The expression of MC, MΦ, T cells （CD4＋ CD8＋） and TGF-β1 in renal biopsy specimens from 51 cases with LN were detected by using immunohistochemical assay. Results The number of the infiltrated T cells（CD4＋, CD8＋） and CD68＋ macrophage in cases with LN were significantly higher than that in the control group. There was a positive correlation between the expression of MC and renal interstitial fibrosis （P〈0.05）,and between the expression of CD68＋ MΦ, T cells（CD4＋, CD8＋） and renal interstitial inflammation（P〈0.05）. Conclusion MC may be the major participant the in the renal interstitial fibrosis of LN, and TGF-β1 may be the important chemokines for the MC, MΦ and T cells.