摘要
目的建立大豆甾醇提取物中总甾醇的含量测定方法。方法采用分光光度法,以β-谷甾醇为对照品,磷硫铁试剂为显色剂,测定波长为530nm。结果甾醇的吸光度在3h内稳定;β-谷甾醇质量浓度在10.29～51.45μg/mL范围内与吸光度线性关系良好,回归方程为Y=13.586X+0.0214,r=0.9996(n=6),平均加样回收率为102.34%,RSD=1.70%(n=6)。结论该法为大豆总甾醇的定量分析提供了一种简便、快速的测定方法 。
Objective To establish a method for the determination of total sterol in soybean sterol. Methods Spectrophotometry was used. The detection wavelength was at 530 nm, β-sitosterol as the reference substance, ferric chlorine-phosphoril and sulphuric acids （S-P-Fe） reagent was used as the chromogenic agent. Results The absorption value was stable in 3h. The good linearity could be found in the range of 10.29-51.45 μg/mL. The regression equation was Y=13.586 X＋0.021 4, r=0.999 6（n=6）, the average recovery rate was 102.34%, RSD = 1.7% （n =6）. Conclusion The method is simple and rapid for the determination of total sterol in soybean sterol.
出处
《中国药业》
CAS
2010年第8期35-36,共2页
China Pharmaceuticals
关键词
硫磷铁法
分光光度法
大豆甾醇
总甾醇
sulfate - phosphate - ferric method
spectrophotometry
soybean sterol
total sterol
