摘要
目的筛选一枝黄花中有效成分芦丁提取工艺的最优条件及对芦丁进行含量测定。方法采用正交试验法,以提取时间、提取次数、甲醇质量分数为考察因素,以芦丁含量为指标,对提取条件进行优选。采用高效液相色谱(HPLC)法对芦丁含量进行测定。结果最佳提取工艺为提取时间30min,提取次数3次,甲醇质量分数70%。含量测定以乙腈-甲醇-0.4%醋酸溶液(16:8:76)为流动相,流速为1.0mL/min,检测波长为360nm,线性范围为0.6876~1.6044μg(r=0.9995),平均回收率为99.27%,RSD为0.69%(n=6)。结论优选的工艺可行,具有操作性。用HPLC法测定含量简便、灵敏、准确。
Objective To optimize the extracting technology of the effective ingredient rutin from Solidago decurrens Lour and to establish its quality control. Methods The orthogonal test was used, with the extracting time, extracting times and methanol concentration as the factors, the content of rutin as the index for screening the extracting condition. Rutin content was determined by the HPLC methods. Results The best optimized extracting procedure was: each time 30 min,extracting for 3 times and 70% methanol. The Diamonsil C18 column was used with the mobile phase of CH3CN- methanol-0.4% acetic acid aqueous solution(16 : 8 : 76),the detection wavelength was 360 nm,the flow rate was 1.0 mL/min. The good linear range of rutin was within 0. 687 6- 1. 6044μg, r=0. 999 5. The average recovery rate was 99.27% and RSD was 0. 69% (n =6). Conclusion The optimized technology is feasible and easily operative. The HPLC determination method for the rutin content is simple,sensitive and accurate.
出处
《中国药业》
CAS
2010年第8期49-50,共2页
China Pharmaceuticals